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single cell RNA sequencing on CRISPR modified cells

Physical
Pricing/Discount Options: Call #2
Unique Identifier: 59a582f8-243a-4b12-b5b9-0bc2a4e632bf

Service Description

The Units: CRISPR Functional Genomics (CFG) (https://www.scilifelab.se/units/crispr-functional-genomics/) and Eukaryotic Single Cell Genomics (ESCG) (https://www.scilifelab.se/units/eukaryotic-single-cell-genomics/) Both infrastructures are part of Science for Life Laboratory (SciLifeLab) which is an academic collaboration between Swedish universities (including Karolinska Institutet) and a national research infrastructure with a focus on life science.

Both units, CFG and ESCG, have designed a pipeline of CRISPR pooled screens together with single cell RNASeq readout (Perturb-Seq, CROP-Seq). This allows to assess gene expression phenotypes at the single cell level following inactivation of a pool of genes, using Cas-9 expressing cells.

Service span the entire pooled screening process, from Cas-cell line generation and library virus creation, via phenotypic selection by FACS or live/dead, to single cell library preparation, NGS and data analysis.

Offerings: Research & Development Scientific & Medical Communication Testing Laboratory (bioanalysis, wet lab, *-omics, etc.)
TEF-Health Use Case Domain: All
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Provider & Contact

Provider Organisation Karolinska Institutet (KI)
Provider Country Sweden
Published Email tef-health@ki.se

Pricing is available to registered users. SMEs receive significant state-aid reductions (GBER) — or, depending on the call, free services during the funded project. Sign in or register to see the price for your organisation.

Operational Details

Service Inputs Biological sample
Service Outputs Sequencing data analysis
Dependencies & Restrictions ethics vote, GDPR, Facility costs, Material Transfer Agreement