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CRISPR gene editing

Physical
Pricing/Discount Options: Call #2
Unique Identifier: b258d992-e612-401d-b67c-0e1085c40c45

Service Description

The Unit: CRISPR Functional Genomics (CFG) (https://www.scilifelab.se/units/crispr-functional-genomics/) The infrastructure is part of Science for Life Laboratory (SciLifeLab) which is an academic collaboration between Swedish universities (including Karolinska Institutet) and a national research infrastructure with a focus on life science.

We offer a wide array of CRISPR applications, ranging from precision genome engineering in cell lines to massively parallel genetic perturbations for genetic screening and unbiased discovery. As a SciLifeLab national infrastructure unit, we strive to provide services at the cutting edge of technological innovation.

Services span the entire pooled screening process, from Cas-cell line generation and library virus creation, via phenotypic selection by FACS or live/dead, to gDNA and NGS library preparation, NGS, and data analysis.

Example of services provided: Precision gene-editing and functional genomic screens using various CRISPR-systems: - Cas-expressing cell line generation (Cas9, nCas9, dCas9, dCas9 fusions, Cas12a, Cas13d) - CRISPR-ko, CRISPR-inhibition, CRISPR-activation screens - Multiplexed Cas12a screens - Base-editor screens - RNA targeting screens with Cas13d.

We are constantly innovating and are developing base-editing approaches for mutagenesis screens to study protein-drug interactions, as well as optical pooled screens for complex phenotypic readouts.

Offerings: Research & Development Scientific & Medical Communication Testing Laboratory (bioanalysis, wet lab, *-omics, etc.)
TEF-Health Use Case Domain: All
Provider Logo Service Logo

Provider & Contact

Provider Organisation Karolinska Institutet (KI)
Provider Country Sweden
Published Email tef-health@ki.se

Pricing is available to registered users. SMEs receive significant state-aid reductions (GBER) — or, depending on the call, free services during the funded project. Sign in or register to see the price for your organisation.

Operational Details

Service Inputs Biological sample
Service Outputs Sequencing data analysis
Dependencies & Restrictions ethics vote, GDPR, Facility costs, Material Transfer Agreement

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