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Single cell RNA sequencing (scRNA-seq)

Physical
Pricing/Discount Options: Call #2
Unique Identifier: ae512d86-8c6a-4c81-bd61-90e4b52c7499

Service Description

The Unit: Eukaryotic Single Cell Genomics (https://www.scilifelab.se/units/eukaryotic-single-cell-genomics/) The infrastructure is part of Science for Life Laboratory (SciLifeLab) which is an academic collaboration between Swedish universities (including Karolinska Institutet) and a national research infrastructure with a focus on life science. Single-cell genomics technologies are rapidly advancing and have proven to give new insights into cell type discovery and in the characterization of heterogeneity in tumors as well as in normal tissue. The Eukaryotic Single Cell Genomics (ESCG) unit aims at providing high-throughput single cell transcriptomics services through a streamlined and complete single-cell RNA sequencing service. The user provide us with single cells, we process the samples, sequence and deliver annotated gene expression data. • Study heterogeneity within putatively homogeneous cell populations • Unbiased discovery of cell types in complex tissues • Characterizing the cellular and genetic composition of tumors

The service: Single cell RNA sequencing (scRNA-seq) Depending on your sample and your need, we can offer single-cell RNA sequencing using SMART-Seq3 or 10X Genomics methods on live cells, and 10X Genomics FLEX assay on fixed cells (recommended for users outside Stockholm area). SMART-Seq3 alows full-length transcript information at a higher sensitivity compared to 10X Genomics technology (10X Genomics: generates data starting from 3' or 5' end of your transcript of interest). However, 10X Genomics has a higher cell throughput and lower cost per cell compared to Smart-seq3. Sequencing equipment: Illumina NextSeq or NovaSeq platforms. ESCG provides access to technology, end-to-end support from help with project planning, quality check of your sample, cDNA library preparation to analysis and associated bioinformatics support.

Prospective Use case: Single cell transcriptomics can be used in the clinic to understand heterogeneity of cancer sub-clonal population, and potentially to follow how these different sub-clones respond differently to various therapeutic treatments. Therefore, AI algorithms trained on such datasets could help clinical decision-making process for molecular-targeted therapies.

Offerings: Research & Development Scientific & Medical Communication Testing Laboratory (bioanalysis, wet lab, *-omics, etc.)
TEF-Health Use Case Domain: all
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Provider & Contact

Provider Organisation Karolinska Institutet (KI)
Provider Country Sweden
Published Email tef-health@ki.se

Pricing is available to registered users. SMEs receive significant state-aid reductions (GBER) — or, depending on the call, free services during the funded project. Sign in or register to see the price for your organisation.

Operational Details

Service Inputs Biological sample
Service Outputs Sequencing data analysis
Dependencies & Restrictions ethics vote, GDPR, Facility costs, Material Transfer Agreement